Composite

Part:BBa_K3730014

Designed by: Guanrui Huang   Group: iGEM21_ZJU-China   (2021-09-07)


Type 5 Adenovirus E1A gene + designed 3'UTR

This is a combined sequence which we insert a sequence designed by us to the 3' end of the virus E1A gene.

3UTR with miRNA combining sequence: BBa_K3730013

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 780
    Illegal PstI site found at 1213
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 999
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 780
    Illegal PstI site found at 1213
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 780
    Illegal PstI site found at 1213
    Illegal NgoMIV site found at 252
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None